Ключевые слова
highly pathogenic
birds
molecular research
avian influenza
Как цитировать
Аннотация
Introduction The H5N1 avian influenza virus is one of the most virulent antigenic combinations, including those with zoonotic pathogenic potential. As the number of avian influenza outbreaks has increased globally, a corresponding rise in outbreaks caused by this virus has also been recorded in the Republic of Moldova.
Materials and methods The descriptive study employed epidemiological, clinical and molecular biology (RT-PCR) research methods. The research material consisted of carcasses of domestic and wild birds, as well as blood serum samples collected from various regions of the republic. Identification of the influenza virus in the research material was carried out at the National Reference Laboratory Public Institution National Center for Animal, Plant Health and Food Safety (P.I. NCAPHFS) and the EU Reference Laboratory (EURL, IZSVe). The following diagnostic kits were used for virus identification: the "IndiSpin® Pathogen Kit" (QIAGEN® GmbH for INDICAL BIOSCIENCE) and the "Virotype® Influenza A RT-PCR Kit" (Germany).
Results The molecular structure of HPAI A(H5N1) viruses isolated from the Republic of Moldova demonstrated the presence of genetic mutations in the PB2 protein (E627K and/or D701N) associated with viral adaptation to mammalian hosts and isolates with European H5 viruses belonging to the EA-2021-I, EA-2022-CH, EA-2022-AB, and EA-2023-DB genotypes.
Conclusions The molecular characterization of HPAI A(H5N1) isolated from birds in the Republic of Moldova demonstrated the presence of genetic mutations in the PB2 protein and in the hemagglutinin protein (S133A, S154N, and T156A), which are associated with adaptation to human-type receptors.
Библиографические ссылки
1. Alexander DJ. An overview of the epidemiology of avian influenza. Vaccine 2007;26;25(30):5637-44. https://doi.org/10.1016/j.vaccine.2006.10.051
2. Sturm-Ramirez KM, et al. Reemerging H5N1 influenza viruses in Hong Kong in 2002 are highly pathogenic to ducks. J. Virol. 2004;78,4892–4901. https://doi.org/10.1128/jvi.78.9.4892-4901.2004
3. Tong S, Zhu ., Li Y., et all. New world bats harbor diverse influenza A virus. 2013; PLoS Pathog 9: e1003657. https://doi.org/10.1371/journal.ppat.1003657
4. Wu Y, Wu Y, Tefsen B, Shi Y, Gao GF. Bat-derived influenza-like viruses H17N10 and H18N11. Trends Microbiol. 2014;22:183–191. https://doi.org/10.1016/j.tim.2014.01.010
5. Li Q, Sun X, Li Z, et al. Structural and functional characterization of neuraminidase-like molecule N10 derived from bat influenza A virus. Proc Natl Acad Sci USA. 2012; (46):18897–18902. https://doi.org/10.1073/pnas.1211037109.
6. Zhu X, Yu W, et al. Hemagglutinin homologue from H17N10 bat influenza virus exhibits divergent receptor-binding and pH-dependent fusion activities. Proc Natl Acad Sci USA. 2013; 110(4):1458-63. https://doi.org/10.1073/pnas.1218509110
7. Chen H, Deng G, et al. The evolution of H5N1 influenza viruses in ducks in southern China. Proc. Natl. Acad. Sci. USA, 2004; 101(28):10452-7. https://doi.org/10.1073/pnas.0403212101.
8. Xu X, Subbarao K, Cox NJ, Guo Y. Genetic characterization of the pathogenic influenza A/goose/Guandong/1/96 (H5N1) virus: Similarity of its hemagglutinin gene to those of H5N1 viruses from the 1997 outbreaks in Hong Kong. Virology 1999; 261:15–19. https://doi.org/10.1006/viro.1999.9820
9. Chen H, Smith G, Zhang Y, et al. Avian flu: H5N1 virus outbreak in migratory waterfowl. Nature. 2005;436(7048):191-2. https://doi.org/10.1038/nature03974.
10. Alexander D., Brown I. History of highly pathogenic avian influenza. Rev. Sci. Tech. 2009;28,19–38. https://doi.org/10.20506/rst.28.1.1856.
11. Meade PS, Bandawane P, et. al. Detection of clade 2.3.4.4b highly pathogenic H5N1 influenza virus in New York City. J. Virol. 2024;2024.04.04.588061. https://doi.org/10.1101/2024.04.04.588061.
12. World Health Organization. Cumulative Number of Confirmed Human Cases for Avian Influenza A (H5N1) Reported to WHO, 2003–2024, 3 May 2024. Accessed August 7th, 2025: https://www.who.int/publications/m/item/cumulative-number-of-confirmedhuman-cases-for-avian-influenza-a(h5n1)-reported-to-who--2003-2024-3-may-2024
13. Agenția Națională pentru Siguranța Alimentelor. Direcția sănătatea și bunăstarea animalelor. Analiza de risc pentru influența (gripa) aviară în Republica Moldova, 2 decembrie 2024. Accessed August 18th, 2025: https://www.ansa.gov.md/sites/default/files/documents/Aparatul%20central/DSBA/2025/Analiza%20riscului%20pentru%20GA%20%20PDF.pdf
14. Suttie A, Deng YM, et al. Inventory of molecular markers affecting biological characteristics of avian influenza A virus. Virus Genes. 2019;55(6):739-768. https://doi.org/10.1007/s11262-019-01700-z.
15. World Health Organization. Influenza (avian and other zoonotic). Accessed August 15th, 2025: https://www.who.int/news-room/fact-sheets/detail/influenza-(avian-and-other-zoonotic.
16. Updated joint FAO/WHO/WOAH public health assessment of recent influenza A(H5) virus events in animals and people, Assessment based on data as of 18 November 2024, 20 noiembrie 2024. Accessed August 25th, 2025: https://www.woah.org/app/uploads/2024/12/cleared-2024-12-10-fao-woah-who-h5n1-assessment-woah-fao.pdf.
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